il 1 receptor antagonist il 1ra  (R&D Systems)

Journal: Journal of Clinical Medicine

Article Title: Efficacy of Anti-Interleukin-1 Therapeutics in the Treatment of Knee Osteoarthritis: A Systematic Review and Meta-Analysis of Randomized Controlled Trials from the Years 2000 to 2023

doi: 10.3390/jcm13102859

Figure Lengend Snippet: Characteristics of the studies.

Article Snippet: 8 , Chevalier 2009 [ ] , Muliticentre RCT , France , Amgen Inc , Anakinra (IL-1 Ras) , Single IA , 12 , 50 mg , 1 , 34 , 69.

Techniques: Control

Journal: Molecular Pain

Article Title: Rapid cleavage of IL-1β in DRG neurons produces tissue injury-induced pain hypersensitivity

doi: 10.1177/17448069241285357

Figure Lengend Snippet: (a) The plantar incision induced a reduction in mechanical threshold against von Frey stimulation. Intrathecal IL-1RA administration significantly inhibited the reduction of the mechanical threshold for 6 h after the treatment. (b) The plantar incision induced a reduction in latency time against thermal stimulation. Intrathecal IL-1RA administration significantly inhibited reduction in the thermal pain latency for 6 h after the treatment. Data were analyzed by repeated measures two-way ANOVA, with Bonferroni’s multiple comparison test comparing pre- and post-treatment time points for each group ( # p < .05, ## p < .01, ### p < .001), and Bonferroni’s multiple comparison test comparing both groups at each time point (* p < .05, ** p < .01, *** p < .001). The data in the graphs are presented as the mean ± SEM ( n = 6 in each group). i.t.: intrathecal administration. Intrathecal administration of IL-1β induced reduction of the mechanical threshold against von Frey stimulation (c) and reduction of latency time against thermal stimulation (d). Data were analyzed by repeated measures two-way ANOVA, with Bonferroni’s multiple comparison test comparing pre- and post-treatment time points for each group ( # p < .05, ## p < .01) and Bonferroni’s multiple comparison test comparing both groups at each time point (* p < .05, ** p < .01). The data in the graphs are presented as the mean ± SEM ( n = 6 in each group).

Article Snippet: Mice were randomly assigned to saline and IL-1 RA (500 ng/5 μL, diluted in saline, Recombinant Human IL-1ra/IL-1F3, #280-RA/CF, R&D Systems, Inc. Minneapolis, MN 55413) groups ( n = 6 per group).

Techniques: Comparison

Journal: Cell reports

Article Title: Jedi-1/MEGF12-mediated phagocytosis controls the pro-neurogenic properties of microglia in the ventricular-subventricular zone

doi: 10.1016/j.celrep.2023.113423

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat #280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold.

Techniques: Recombinant, Marker, In Situ, RNA Sequencing, Knock-Out, Mutagenesis, Real-time Polymerase Chain Reaction, Software

Journal: Aging (Albany NY)

Article Title: Exosome-coated polydatin nanoparticles in the treatment of radiation-induced intestinal damage

doi: 10.18632/aging.204882

Figure Lengend Snippet: exo-PD acts on intestinal cells via the PI3K-AKT pathway as determined by phosphorylated protein profiling. ( A , B ) Differential proteins and differential loci derived from proteomic and phosphorylated proteomic modification assays. ( C ) STING protein interactions. Red represents phosphorylation regulation, blue represents PI3K-AKT signalling pathway and green represents intracellular membrane-bound organelles. ( D ) Effect of PD, exo and ex-PD on protein expression of PI3K/AKT and γ-H2AX in mouse intestine after 24 h of IR. ( E ) Western blot of IL-1α and IL-6 in mouse intestine after exo-PD treatment. ( F ) Expression of PI3K and p-AKT after treatment with different conditions following the addition of AKT inhibitors.

Article Snippet: The list of antibodies used is as follows: p-PI3K: Cell Signaling (17366s); PI3K: Cell Signaling (4263s); p-AKT: Cell Signaling (4058s); AKT: Cell Signaling (9272s); γ-H2AX: Cell Signaling (25775); NF-kappaB: Epitomics (2229-1); IL-1 Ra: Abcam (ab124962); IL-6: Abcam (ab259341); β-actin: Abcam (ab133626).

Techniques: Derivative Assay, Modification, Expressing, Western Blot